Figure 13.

Examination by electron microscopy of NE and NPC morphology in wt and nup57-E17 cells. NUP57 (SWY809) and nup57-E17 (SWY1586) cells were grown at either 23°C or shifted to growth at 37°C for 4 h before processing for thin section electron microscopy. Arrows point to NPCs with apparently wt structure; black arrowheads point to NPC-like electron dense masses that may not span the NE lumen between the inner and outer membranes; open arrowheads point to NE herniations. (A) nup57-E17 at 23°C. (B–D, G, H) nup57-E17 cells at 37°C. (E) wt at 23°C. (F) wt at 37°C. n, nucleus; c, cytoplasm. Bars, 500 nm (A–F); 250 nm (G and H).