Figure 2.

Schematic diagram for a fluorescence-based strategy to isolate NPC assembly mutants. Strains expressing GFP-Nup49p were selected by FACS and screened by microscopic inspection of GFP-NPC fluorescence patterns. Candidate mutants were tested for plasmid-dependent complementation and for growth phenotypes. We predicted that at least three different classes of mutants would be identified: Class I, NPC clustering; Class II, fewer total NPCs per nucleus, and Class III, wt NPC number, but each NPC with a decreased amount of GFP-Nup incorporated.