Figure 3.

Two classes of NPC mutants were isolated in the GFP-Nup49p fluorescence-based strategy: NPC clustering mutants and dim mutants. Direct fluorescence microscopy analysis of GFP-Nup49p localization in wt, nup133Δ, and two representative mutant strains isolated in the FACS-based screen. The strains were grown in SD media lacking leucine: wt (SWY894), nup133Δ clustering (SWY1667), clustering-Class I (nup120-C36 mutant, SWY1710), Dim-Class III (nup57-E17 mutant, SWY1708). Within each column, photographs were exposed and printed for identical times. DAPI staining for each respective field is shown on the left. Bar, 10 μm.