Figure 4.

Expression of NUP57 from a plasmid complements the temperature sensitivity of the nup57-E17 mutant. (A) Strains were grown on SD plates lacking leucine for 4 d at 23 or 37°C: wt (SWY894), nup57-E17 with pRS315 (SWY1708), nup57-E17 + pSW806/NUP57 (SWY1709). NUP57 expression restores growth to the nup57-E17 strain at 37°C. (B) Growth analysis of wt, nup57-E17, and nup57-E17 + NUP57 cells at 23 and 37°C. Aliquots of cells growing at early logarithmic phase were counted at various time points after shift to 37°C or continued growth at 23°C. Cell counts are expressed as cell number per ml of culture.