Table 1.
Yeast strains used in this study
| Strain | Genotype | Derivation |
|---|---|---|
| CHY104 | Mata ura3-52 his3Δ200 leu2Δ1 NUP120::HIS3 | Heath et al., 1995 |
| L40 | Mata his3Δ200 trp1-901 leu2-3,112 ade2 LYS2::(LexAop)4-HIS3 URA3::(LexAop)8-LacZ | S. Hollenberg |
| nic96-1 | Mata ade2 trp1 leu2 ura3 HIS3::nic96 (pUN100-LEU2-nic96ts [P332L;L260P]) | Zabel et al., 1996 |
| SWY422 | Mata/Matα ade2-1/ade2-1 ura3-1/ura3-1 his3-11,15/his3-11/15 trp1-1/trp1-1 leu2-3,112/leu2-3,112 can1-100/can1-100 nup133Δ::HIS3/nup133 | Chromosomal integration of HIS3 at NUP133 locus |
| SWY423 | Matα ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 nup133Δ::HIS3 | Segregant of SWY422 |
| SWY458 | Mata ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 ade2-1:ADE2-URA3 | Bucci and Wente, 1997 |
| SWY459 | Matα ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 ade2-1:ADE2-URA3 | Bucci and Wente, 1997 |
| SWY518 | Mata ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 ade2-1:ADE2 | Bucci and Wente, 1997 |
| SWY519 | Matα ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 ade2-1:ADE2 | Bucci and Wente, 1997 |
| SWY595 | Mata/MATα ura3-1/ura3-1 his3-11,15/his3-11,15 trp1-1/trp1-1 leu2-3,112/leu2-3,112 can1-100/can1-100 ade2-1:ADE2/ade2-1:ADE2 | Bucci and Wente, 1997 |
| SWY729 | Matα ade2-1:ADE2-URA3 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 nup133Δ::HIS3 | Transformation of SWY423 with the integrating vector pSW286 (Bucci and Wente, 1997) |
| SWY734 | Mata ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 nup49-1::URA3 nup49ΔGLFG::GFP-S65T-TRP1 | Bucci and Wente, 1997 |
| SWY737 | Matα ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 nup49-1::URA3 | Bucci and Wente, 1997 |
| SWY760 | Matα ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 ade2-1:ADE2-URA3 pSW242 (LEU2) | SWY459 transformed with pSW242 |
| SWY809 | Mata ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 ade2-1:ADE2 nup49-1::URA3 nup49ΔGLFG::GFP-S65T-TRP1 | Bucci and Wente, 1997 |
| SWY811 | Matα ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 ade2-1:ADE2 nup49-1::URA3 nup49ΔGLFG::GFP-S65T-TRP1 | Segregant of diploid created by crossing SWY737 with SWY518 |
| SWY828 | Mata ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 ade2-1:ADE2-URA3 nup49-1::URA3 nup49ΔGLFG::GFP-S65T-TRP1 nup133Δ::HIS3 | Segregant of diploid created by crossing SWY729 with SWY734 |
| SWY894 | Mata ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 ade2-1:ADE2 nup49-1::URA3 nup49ΔGLFG::GFP-S65T-TRP1 pRS315 (LEU2) | SWY809 transformed with pRS315 |
| SWY1186 | Mata ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 lys2 gle1-4 | Murphy and Wente, 1996 |
| SWY1226 | Matα ade2-1 ura3-1 his3-11,15 leu2-3,112 trp1-1 can1-100 GLE2Δ::HIS3 | Murphy et al., 1996 |
| SWY1427 | Mata/Matα ura3-1/ura3-1 his3-11,15/his3-11,15 trp1-1/trp1-1 leu2-3,112/leu2-3,112 can1-100/can1-100 ade2-1:ADE2/ade2-1:ADE2 NUP82-GFP-F64L, S65T-kanR/NUP82 | SWY595 transformed with the PCR product created with oligos NUP82G and NUP82K using pSW639 as template |
| SWY1441 | Mata ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 ade2-1:ADE2 NUP82-GFP-F64L,S65T-kanR | Segregant of SWY1427 |
| SWY1511 | Mata ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 ade2-1:ADE2 NUP82-GFP-F64L,S65T-kanR pRS425 (LEU2) | SWY1441 transformed with pRS425 |
| SWY1586 | Matα ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 ade2-1:ADE2 nup57-E17 nup49-1::URA3 nup49ΔGLFG::GFP-S65T-TRP1 | Segregant of a backcross between SWY811 and the nup57-E17 clone obtained from mutagenesis of SWY809 |
| SWY1587 | Matα ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 ade2-1:ADE2 nup57-E17 | Segregant of a backcross between SWY760 and the nup57-E17 clone obtained from mutagenesis of SWY809 |
| SWY1601 | Mata ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 ade2-1:ADE2-URA3 pRS315 (LEU2) | SWY458 transformed with pRS315 |
| SWY1667 | Mata ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 ade2-1:ADE2-URA3 nup49-1::URA3 nup49ΔGLFG::GFP-S65T-TRP1 nup133Δ::HIS3 pRS315 (LEU2) | SWY828 transformed with pRS315 |
| SWY1693 | Mata/Matα ura3-1/ura3-1 his3-11,15/his3-11,15 trp1-1/trp1-1 leu2-3,112/leu2-3,112 can1-100/can1-100 ade2-1:ADE2/ade2-1:ADE2 GFP-S65T-NIC96-HIS3/NIC96 | SWY595 transformed with AFlII-digested pSW950 |
| SWY1695 | Mata ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 ade2-1:ADE2 GFP-S65T-NIC96-HIS3 | Segregant of SWY1693 |
| SWY1705 | Mata ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 ade2-1:ADE2 nup49-1::URA3 nup49ΔGLFG::GFP-S65T-TRP1 gle2-C18 pRS315 (LEU2) | Mutagenesis of SWY809 and transformation with pRS315 |
| SWY1706 | Mata ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 ade2-1:ADE2 nup49-1::URA3 nup49ΔGLFG::GFP-S65T-TRP1 gle2-C18 pSW406 (LEU2) | Mutagenesis of SWY809 and transformation with pSW406 |
| SWY1707 | Matα ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 ade2-1:ADE2 nup49-1::URA3 nup49ΔGLFG::GFP-S65T-D66-TRP1 pRS315 (LEU2) | Mutagenesis of SWY811, one backcross to SWY809, and transformation with pRS315 |
| SWY1708 | Matα ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 ade2-1:ADE2 nup57-E17 nup49-1::URA3 nup49ΔGLFG::GFP-S65T-TRP1 pRS315 (LEU2) | SWY1586 transformed with pRS315 |
| SWY1709 | Matα ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 ade2-1:ADE2 nup57-E17 nup49-1::URA3 nup49ΔGLFG::GFP-S65T-TRP1 pSW806 (LEU2) | SWY1586 transformed with pSW806 |
| SWY1710 | Matα ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 ade2-1:ADE2 nup49-1::URA3 nup49ΔGLFG::GFP-S65T-TRP1 nup120-C36 pRS315 (LEU2) | Mutagenesis of SWY811 and transformation with pRS315 |
| SWY1711 | Matα ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 ade2-1:ADE2 nup49-1::URA3 nup49ΔGLFG::GFP-S65T-TRP1 nup120-C36 pCH4 (LEU2) | Mutagenesis of SWY811 and transformation with pCH4 |
| SWY1717 | Mata/Matα ura3-1/ura3-1 his3-11,15/his3-11,15 trp1-1/trp1-1 leu2-3,112/leu2-3,112 can1-100/can1-100 ade2-1:ADE2/ade2-1:ADE2 nsp1ΔN::GFP-S65T-HIS3/NSP1 | SWY595 transformed with SpeI-digested pSW956 |
| SWY1722 | Mata ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 ade2-1:ADE2 nup57-E17 GFP-S65T-NIC96-HIS3 | Segregant of a diploid created by crossing SWY1695 and SWY1586 |
| SWY1728 | Matα ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 ade2-1:ADE2 nsp1ΔN:GFP-S65T-HIS3 | Segregant of SWY1717 |
| SWY1729 | Mata ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 ade2-1:ADE2 nsp1ΔN::GFP-S65T-HIS3 | Segregant of SWY1717 |
| SWY1736 | Matα ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 ade2-1:ADE2 nup57-E17 nsp1ΔN::GFP-S65T-HIS3 | Segregant of a diploid created by crossing SWY1729 and SWY1586 |
| SWY1744 | Matx ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 ade2-1:ADE2 nup57-E17 NUP82-GFP-F64L,S65T-kanR pRS425 (LEU2) | Segregant of a diploid created by crossing SWY1511 and SWY1586 |
| SWY1824 | Mata ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 ade2-1:ADE2-URA3 nup133Δ::HIS3 GFP-S65T-NIC96-HIS3 | Segregant of a diploid created by crossing SWY729 and SWY1695 |
| SWY1860 | Mata ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 ade2-1:ADE2 nup49-1::URA3 nup49ΔGLFG::GFP-S65T-TRP1 HIS3::nic96 (pUN100-LEU2-nic96ts [P332L;L26P]) | Segregant of a diploid created by crossing SWY811 and nic96-1 |