Table 3.
C-terminal truncation of Nup57p results in a decreased two-hybrid interaction with Nup49p.
| Lex ABD | GAL4AD | β-Galactosidase activity |
|---|---|---|
| Nup57p | Nup49p (242-493) | 58.2 ± 2.8 |
| Nup57p (1-515) | Nup49p (242-493) | 1.0 ± 0.6 |
| Orc2p | Nup49p (242-493) | 0.1 ± 0.2 |
| Nup57p | Snf4p | 0.6 ± 0.6 |
| Nup57p | — | 0.9 ± 1.2 |
L40 was cotransformed with plasmids containing the indicated activation domain (pSW921, pSE1111) and DNA-binding domain constructs (pSW924, pSW925, pCH428) and grown in SD lacking tryptophan and leucine. Each β-galactosidase assay was completed in triplicate. Plasmids expressing Gal4pAD-Snf4p (Yang et al., 1992) and LexABD-Orc2p (Hardy, 1996) were used to determine the specificity of the Nup49p-Nup57p interaction.