Table 2.
Primers used for qRT-PCR
| Transcript type | Accession number | Primer orientation | Primer sequence (5′ to 3′) |
|---|---|---|---|
| Zinc finger protein F35 | TC301590 | forward | TCAGCTTCTGGTGCTGGT |
| reverse | CGCTCTTCTGCAGCAACT | ||
| SCARECROW-like protein | TC295581 | forward | TCCTACAACGCACCCTTC |
| reverse | CGTTGATTGCCTCTCGAC | ||
| TA1 protein-like, partial (8%) | CO453792 | forward | TGCACGATGTGAAGCAAC |
| reverse | ATTATTTCGGCGGTGGAT | ||
| DNA-binding protein RAV1-like | TC285041 | forward | AAGCGAAGCAAGCATGAG |
| reverse | CCGTGGCAAACAAGAGAG | ||
| WRKY transcription factor 28 | TC300389 | forward | ATGCCTGGATTGCTGAAG |
| reverse | TTCACCCGGAGAGATTTG | ||
| WRKY transcription factor 32 | TC293926 | forward | CCTCTCGAGTGCTTTGGA |
| reverse | AAAAGCCCTGTTGGCTTC | ||
| ZmMybst1 | TC312601 | forward | TCCTTTCTGGCCAAATCA |
| reverse | GACACCGTTTCCTGACCA | ||
| DNA excision repair protein | TC295292 | forward | TCTTTTGGGTCGCTCTCA |
| ERCC-1 | reverse | GAAGGTTGGCCTTTTGCT | |
| BTH-induced ERF transcriptional | TC305954 | forward | CACCCACAGCTCCAACAT |
| factor 3 | reverse | GGGCTTAGGCTCCTTTCA | |
| DNA gyrase subunit B | BI992346 | forward | TCAATCCTTGCCTCCAGA |
| reverse | AGCAGCGAAGTGTGTGGT | ||
| Abscisic acid insensitive 5 | TC301287 | forward | GGCCAGGTGTTTTCCTCT |
| reverse | TCTCCAGCTCGTTGGTGT | ||
| Thioredoxin-like gene | AW927774 | forward | GGACCAGAAGATTGCAGAAG |
| reverse | CAGCATAGACAGGAGCAATG |
a Primers specifically designed to detected decreased transcript accumulation mediated by RNAi transformation