Figure 6.

KIBRA k/d in podocytes. (A) For determination of the KIBRA k/d efficiencies of different shRNA, luciferase assays using the psiCHECK2 (Promega) system were performed. shRNA 1 and 2 resulted in approximately 60 to 65% k/d of the reporter luciferase (Supplemental Figure S3). (B) The expression level for PATJ, synaptopodin, aPKCζ, and rhoA is not changed in KIBRA k/d podocytes (lane 2) compared with control cell lines (empty vector–transduced cells, lane 1), emphasizing that the migration impairment is caused by the decreased endogenous KIBRA expression.