Table 2.
TR J gene reverse primers
| OLIGO NAME | OLIGO SEQUENCE | J GENES OPTIMALLY RECOGNIZED |
| ALPHA | ||
| TRAJ6rev | CGG ATG AAC AAT AAG GCT GGT TC | J6 |
| TRAJ10rev | GAG TTC CAC TTT TAG CTG AG | J10 |
| TRAJ11rev | TGG AGA GAC TAG AAG CAT AG | J11 |
| TRAJ12rev | TGG ACT GAC CAG MAG TCK GG | J12, J8 |
| TRAJ13rev | TGG GAT GAC TTG GAG CTT TG | J13 |
| TRAJ15rev | GGA ACT CAC TGA TAG GTG GG | J15 |
| TRAJ16rev | AAG ATC CAC CTT TAA CAT GG | J16 |
| TRAJ17rev | TGG TTT AAC TAG CAC CCT GG | J17 |
| TRAJ20rev | TGC TCT TAC AGT TAC TGT GG | J20 |
| TRAJ21rev | TGG TTT TAC ATT GAG TTT GG | J21 |
| TRAJ22rev | AGG CCA RAC AGT CAA YTG WGT | J22, J18 |
| TRAJ23rev | GGG TTT CAC AGA TAA CTC CG | J23 |
| TRAJ25rev | TGG TAT GAC CAC MAC YTG GKT | J25, J7 |
| TRAJ26rev | GGG CAG CAC GGA CAA TCT GG | J26 |
| TRAJ27rev | TGG CTT CAC AGT GAG CGT AG | J27 |
| TRAJ29rev | TGC TTT MAC ARA WAG TCT TGT | J29, J9 |
| TRAJ30rev | GGG GAG AAT ATG AAG TCG TG | J30 |
| TRAJ31rev | GGG CTT CAC CAC CAG CTG AG | J31 |
| TRAJ32rev | TGG CTG GAC AGC AAG CAG AG | J32 |
| TRAJ33rev | TGG CTT TAT AAT TAG CTT GG | J33 |
| TRAJ34rev | TGG AAA GAC TTG TAA TCT GG | J34 |
| TRAJ37rev | TGG TTT TAC TTG TAA AGT TG | J37 |
| TRAJ38rev | CGG ATT TAC TGC CAG GCT TG | J38 |
| TRAJ40rev | TGC TAA AAC CTT CAG CCT GG | J40 |
| TRAJ41rev | GGG TGT GAC CAA CAG CGA GG | J41 |
| TRAJ42rev | TGG TAT GAC MGA GAG TTT RGT SC | J42, J28 |
| TRAJ44rev | TGG TTG CAC YTG RAG TCT TGT TC | J44, J5 |
| TRAJ45rev | GGG CTG GAT GAT TAG ATG AG | J45 |
| TRAJ46rev | GGG CCT AAC TGC TAA ACG AG | J46 |
| TRAJ47rev | GGA CTT GAC TCT CAG AAT GG | J47 |
| TRAJ48rev | TGG CCG GAT GST GAG TCT KGT YC | J48, J3 |
| TRAJ36rev | GGG AAT AAY GGT GAG TCT YGT TC | J48, J36 |
| TRAJ49rev | GGG TTT GAC CRT YAA MCT TGT | J49, J39 |
| TRAJ50rev | AGG TTT TAC TGA TAA KCT TGT CC | J50, J14 |
| TRAJ52rev | TGG ATG GAC AGT CAA GAT GG | J52 |
| TRAJ53rev | TGG ATT CAC GGT TAA GAG AG | J53 |
| TRAJ54rev | TGG GTG TAY AGY CAG CCT GGT YC | J54, J4 |
| TRAJ56rev | TGG TCT AAC AC TCA GAG TTA | J56 |
| TRAJ57rev | TGG TTT TAC TGT CAG TYT SG | J57, J43 |
| BETA | ||
| TRBJ1rev | TGT GAC YGT GAG YCT GGT GC | J1-1, J2-7 |
| TRBJ2rev | TGT CAC RGT KAR CCT GGT CC | J1-2, J1-6 |
| TRBJ3rev | TAC AAC AGT GAG CCA ACT TC | J1-3 |
| TRBJ4rev | CAG CAC WGA GAG CYG GGT YC | J1-4, J2-4 |
| TRBJ5rev | TAG GAT GGA GAG TCG AGT CC | J1-5 |
| TRBJ2.1rev | TAG CAC TGT SAG CCG KGT SCC TG | J2-1, J2-3 |
| TRBJ2.2rev | CAG AAC CAG GAG TCC TCC GC | J2-2P |
| TRBJ2.6rev | CAG TAC GGT CAG CCT RSW GC | J2-2, J2-6 |
| TRBJ2.5rev | GAG CAC CAG GAG CCG CGT GCC TG | J2-5 |
| GAMMA | ||
| TRGJP1rev | AGG CGA AGT TAC TAT GAG CY | JP1,JP2 |
| TRGJPrev | TGT AAT GAT AAG CTT TGT TC | JP |
| TRGJ1rev | TGT GAC AAC MAG TGT TGT TC | J1,J2 |
| DELTA | ||
| TRDJ1rev | TGG TTC CAC GAT GAG TTG TG | J1 |
| TRDJ2rev | TGG TTC CAC AGT CAC ACG GG | J2 |
| TRDJ3rev | GGG CTC CAC GAA GAG TTT GA | J3 |
| TRDJ4rev | TTG TTG TAC CTC CAG ATA GG | J4 |
List of optimal primer sequence as designed with the TCRAlignment and TCROligo algorithms for the TRAJ, TRBJ, TRDJ and TRGJ genes.