Table 2.

Antibodies used in the study.

Protein	Order number and company	Type	Dilution	Incub. time	Blocking
CD45RB	M0833 (B cells, T cells subsets, monocytes, macrophages and some granulocytes) DAKO A/S.	Mc mouse anti-human	1:200	+4°C o/n	D
CD68	M0876 (macrophages) DAKO A/S, Glostrup, Denmark	Mc mouse anti-human	1:200	+4°C o/n	D
MMP-9	MS-817-P0, NeoMarkers Inc	Mc mouse anti-human	1:400	RT 60 min	D
IL-8	sc-73321, Santa Cruz Biotechnology, Inc	Mc mouse anti-human	1:200	+4°C o/n	A
PAF-R	160600, Cayman chemical,	Mc mouse anti-human	1:350	+4°C o/n	E
S-1 (CD138)	M7228, DakoCytomation,	Mc mouse anti human	1:50	RT 30 min	A
ERα	2nd Gen 08-1149, Zymed Laboratories Inc	Mc mouse anti-human	1:5	+4°C o/n	A
ERβ	Serotec Ltd, MCA1974	Mc mouse anti-human	1:20	+4°C o/n	B
GPR30	LS-A4272, LifeSpan BioSciences	Pc rabbit anti-human	1:300	RT 60 min	C
Ki-67	NCL-Ki67-MM1, Novocastra Laboratories Ltd	Mc mouse anti human	1:200	+4°C o/n	A

Mc = monoclonal; Pc = polyclonal, A = 1.5% normal horse serum (NHS) in PBS for 30 min, B = 1.5% normal horse serum (NHS) in TBS containing 5% bovine serum albumin (BSA) for 45 min, C = 1.5% normal goat serum (NGS) in PBS containing 5% bovine serum albumin (BSA) for 30 min, D = 1.5% normal horse serum (NHS) in PBS for 45 min, E = 1.5% normal horse serum (NHS) in TBS for 45 min.

NHS = normal horse serum (Vector Laboratories, Burlingame, CA).

NGS = normal goat serum (DakoCytomation, Glostrup, Denmark).