Table 3.
Secondary anti-bodies used in the study, their dilution, buffers and incubation time.
| Protein | 2nd Ab | Dilution | Buffer | Incubation |
| CD45RB | biotinylated horse anti-mouse |
1:200 | 1.5% NHS in PBS | 60 min RT |
| CD68 | biotinylated horse anti-mouse |
1:200 | 1.5% NHS in PBS | 60 min RT |
| MMP-9 | biotinylated horse anti-mouse |
1:200 | 1.5% NHS in PBS | 60 min RT |
| IL-8 | biotinylated horse anti-mouse |
1:200 | 1.5% NHS in PBS | 30 minRT |
| PAF-R | biotinylated horse anti-mouse |
1:200 | 1.5% NHS in TBS | 30 min RT |
| S-1 (CD138) |
biotinylated horse anti-mouse |
1:200 | 1.5% NHS in PBS | 30 min RT |
| ERα | biotinylated horse anti-mouse |
1:200 | 1.5% NHS in PBS | 30 minRT |
| ERβ | biotinylated horse anti-mouse |
1:200 | 1.5% NHS in TBS + 5% BSA | 60 min RT |
| GPR30 | biotinylated goat anti-rabbit |
1:200 | 1.5% NGS in PBS + 5% BSA | 30 min RT |
| Ki67 | biotinylated horse anti-mouse |
1:200 | 1.5% NHS in PBS | 30 min RT |
biotinylated horse anti-mouse antibody (Vector, Cat# BA-2000).
biotinylated goat anti-rabbit antibody (Vector, Cat# BA-1000).