Figure 3.

In CT-cells, the blockage of p38 kinase counteracts exogenous calcitonin (CT) stimulatory effect on CD44 variant RNA expression. Triplicate TaqMan RT-PCR experiments. While p38 blockade does not affect the CT-induced decrease in CD44 total, it does abrogate the expected tripling (Fig. 1) in CD44 variant. Conversely, MEK inhibitor moderately reduced total and variant CD44, and this effect was not counteracted by CT, suggesting CT does not act downstream of MEK. *p = 0.02; **p = 0.006 with respect to mock treated controls.