Figure 2.

ATP synthesis in neuroblastoma cells utilising glycolysis or oxidative phosphorylation. The ATP content per mg of total cell protein under different energy substrate restriction conditions was determined spectrophotometrically using a luciferase-based reporter assay. Cells were cultured in □ normal culture medium (DMEM); conditions inhibiting oxidative phosphorylation (DMEM plus rotenone); ■ conditions inhibiting glycolysis (DMEM containing pyruvate but no glucose (PNG media)); conditions inhibiting both oxidative phosphorylation and glycolysis (PNG plus rotenone). BE(2)-C cells produced significantly different (*p < 0.01, #p < 0.05) levels of ATP under conditions inhibiting oxidative phosphorylation or glycolysis compared to that produced in normal culture media.