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Characterization of Xenopus ASAP. Asynchronous XL2 cells were transfected with the EYFP-X-ASAP cDNA and analyzed (A) by immunoblot using an anti-GFP antibody or (B) by immunofluorescence. Cells were fixed in PAF/MTSB and co-stained with the anti α-tubulin (red) and Hoechst 33258 (blue).
