Figure 6.

Overexpression of Cter and Nter ASAP deletion mutants. Forty-eight hours after transfection with 1 μg of EYFP-ASAP-Cter or EYFP-ASAP-Nter, U2-OS cells were analyzed by immunofluorescence (A) and western-blot (B). (A) Cells were fixed with PFA and stained with an anti-α-tubulin (red) and Hoechst dye 33258 (DNA). EYFP signals are in green. (Bar, 10 μm). (B) Immunoblot of EYFP, EYFP-ASAP-FL (full-length), EYFP-ASAP-Cter or EYFP-ASAP-Nter U2-OS transfected cells using an anti-GFP antibody (top); NT: non-transfected cells. The same blot was probed with an anti-α-tubulin to demonstrate equal loading (bottom).