FIG. 5.

Pattern of c-Fos/POMC–and c-Fos/NPY–positive cells after an intragastric water or glucose infusion. c-Fos/POMC–positive cells were quantified in the ARC. Representative figures of c-Fos–positive cells (A) and POMC-positive cells (B) in the ARC. ARC was magnified. The POMC-positive cells showing clear staining in cytoplasm are indicated by white arrows. C: Representative figure of c-Fos/POMC–positive cells showing that POMC-positive cells were not colocalized with c-Fos in the magnified field. c-Fos/NPY–positive cells was quantified in the ARC. D and E: Immunohistochemical representation of cFos and NPY and NPY/cFos-positive cells, as indicated by arrows, in mice infused with water or glucose. F and G: Quantification of NPY and NPY/c-Fos-positive cells in mice infused with water (□) or glucose (▪). Five to six mice per group were studied. (Please see http://dx.doi.org/10.2337/db07-1788 for a high-quality digital representation of this figure.)