FIG. 5.

Role of Akt/PKB phosphorylation by high glucose (HG) on OGG1 expression in MCT cells. A: Representative immunoblot shows a decrease in phospho-Akt (p-Akt) and phospho-tuberin (A) and an increase in OGG1 expression (B) in cells preincubated with 25 μmol/l Akt inhibitor IV before exposure to high glucose for 60 min. Histograms in the bottom panel represent means ± SE of three independent experiments. Significant difference from nontreated cells is indicated by *P < 0.05 and **P < 0.01. NG, normal glucose.