FIG. 6.

Effects of hydrogen peroxide on tuberin and Akt/PKb phosphorylation and OGG1 expression in MCT cells. A–C: Representative immunoblot shows an increase in phospho-Akt (p-Akt) (A) and phospho-tuberin (p-tuberin) (B) and a decrease in OGG1 expression (C) in MCT cells treated with 100 μmol/l H₂O₂. GAPDH was used as loading control. Histograms in the bottom panel represent means ± SE of three independent experiments. Significant difference from nontreated cells is indicated by *P < 0.05 and **P < 0.01. HG, high glucose; NG, normal glucose.