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. 2008 Oct;57(10):2644–2651. doi: 10.2337/db06-0454

FIG. 1.

FIG. 1.

Generation of Tg IRS-1 Ser→Ala mice. A: Serine phosphorylation of IRS-1 protein in skeletal muscle. Serine phosphorylation of IRS-1 and protein expression of IRS-1 were analyzed by Western blotting. All mice were killed at age 16 weeks. The high-fat diet group was fed for 8 weeks. B: Vector construct of the mutated IRS-1 gene (Ser302, Ser307, and Ser612 to Ala mutant) for Tg IRS-1 Ser→Ala and WT IRS-1 gene for Tg IRS-1 WT. C: Comparison of IRS-1 expression in different insulin-targeting tissues. Protein expression of total IRS-1 and actin were analyzed by Western blotting. D: Comparison of IRS-1 expression between Tg IRS-1 Ser→Ala and Tg IRS-1 WT mice. Each graph was expressed as fold difference to their littermates. GAS, Musculus gastrocnemius; QD, Musclus quadriceps; TA, Musculus tibialis anterior; WAT, white adipose tissue.