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miR34a and miR146 expression is increased in pancreatic islets of db/db mice. Pancreatic islets were isolated from four wild-type mice and five diabetic db/db mice. Blood glucose level was 6.8 ± 0.4 mmol/l in control animals and 21.8 ± 1.4 mmol/l in db/db mice (P < 0.001). RNA extracts from each islet preparation were analyzed by quantitative RT-PCR for the expression of the indicated miRNAs. Data are expressed as percent U6 content measured in the same sample.
