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. 2008 Oct;57(10):2728–2736. doi: 10.2337/db07-1252

FIG. 3.

FIG. 3.

miR34a and miR146 expression is induced in a time- and dose-dependent manner by palmitate but not by unsaturated FFAs. A: MIN6B1 cells were cultured for the indicated time in normal medium (control) or in the presence of 1 mmol/l palmitate (palm). After RNA extraction, miR34a and miR146 levels were determined by quantitative RT-PCR. Data are expressed as percent U6 content measured in the same samples. *Conditions significantly different (P < 0.05, n = 3) from control. B: The cells were incubated for 72 h in normal DMEM (control) or in DMEM containing the indicated concentrations of palmitate. miR34a, miR146, and U6 levels were determined by quantitative RT-PCR. The results are expressed as percent U6 content in each sample. *Conditions significantly different (P < 0.05, n = 3) from control. C: The cells were incubated for 72 h in normal medium (control) or in the presence of 0.5 mmol/l palmitate (Palm), 0.5 mmol/l oleate, or 0.5 mmol/l linoleate. miR34a and miR146 levels were determined by quantitative RT-PCR and normalized to the U6 content. *Conditions significantly different (P < 0.05, n = 3) from control.