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. 2008 Oct;57(10):2774–2783. doi: 10.2337/db07-1746

FIG. 4.

FIG. 4.

Effect of kinase inhibitors on Nnat-induced NF-κB activation. A: Western blot analysis of Iκ B-α, Phospho-p65 (P-p65), VCAM-1, β-actin P-p38, P-JNK1/2, P-ERK1/2, and P-AKT protein levels in the presence of kinase inhibitors. IL-6 (B) and SELE (C) mRNA expression in HAECs infected with Ad-Nnat. Effect of kinase inhibitors on Nnat-induced increase in IL-6 and SELE expression levels, normalized to GAPDH. I, no inhibitor; U0, U0126; SB, SB202190; SP, SP600125; LY, LY294002; W, Wortmannin; and IK, IKK-VII. ***P < 0.001, **P < 0.01. Data are representative of three to four experiments. D: Schematic describing Nnat-induced signal transduction in endothelial cells. Inhibitors of PI 3-kinase and p38 completed blocked Nnat-induced NF-κB activation, JNK1/2 inhibition led to a partial block, and inhibition of ERK1/2 increased NF-κB activation.