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. 2008 Oct;57(10):2784–2793. doi: 10.2337/db08-0302

FIG. 6.

FIG. 6.

Inhibitory effects of simvastatin on vitreous-induced MLC phosphorylation and contraction of hyalocyte-containing collagen gels. A: Starved hyalocytes were pretreated with 5 μmol/l simvastatin for 30 min and subsequently treated with or without 400 μl vitreous samples of non-PVD (macular hole) or PVD (PDR and PVR) for 24 h. Total cell lysates were subjected to Western blot analysis with an antibody against p-MLC. Lane loading differences were normalized by reblotting the membranes with an antibody against MLC and GAPDH. B: Signal intensity ratios (p-MLC to GAPDH) were expressed as percentage of control intensity ratio. C: Hyalocytes were embedded in type I collagen gels (n = 4). After starvation and pretreatment with 5 μmol/l simvastatin for 24 h, the collagen gels were stimulated with 400 μl vitreous samples of non-PVD or PVD. Five days after the stimulation, the gels were photographed. D: The diameter of the collagen gels was measured and expressed as percentage of the average diameter of the control group. *P < 0.05.