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Nuclear translocation of nrf2 in human HMEC-1 endothelial cells in vitro activated by sulforaphane. Nuclear fraction (A) and cytosolic fraction (B) immunoblotting for nrf2 (98-kDa band). Densitometric intensity ratios are means ± SD (n = 3). N, 5 mmol/l glucose; H, 30 mmol/l glucose; N+SFN, 5 mmol/l glucose + 4 μmol/l sulforaphane; and H+SFN, 30 mmol/l glucose + 4 μmol/l sulforaphane. *P < 0.05, **P < 0.01, and ***P < 0.001 with respect to N; ○P < 0.05 and ○○P < 0.01 with respect to H.
