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. Author manuscript; available in PMC: 2008 Sep 24.
Published in final edited form as: Glycoconj J. 2008 Mar 26;25(6):581–593. doi: 10.1007/s10719-008-9123-y

Fig. 2.

Fig. 2

SDS-PAGE with Coomassie blue stain (a) and Western blot with antibodies αhLF (b) and αHCP (c) of samples at different purification steps. Lane 1 broad range molecular weight standards, lane 2 rhLF fermentation supernatant, lane 3 SP Sepharose Fast Flow eluant, lane 4 Heparin Sepharose 6 Fast Flow eluant. αHCP antibody against Pichia host cell proteins