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. Author manuscript; available in PMC: 2009 Oct 10.

Published in final edited form as: J Mol Biol. 2008 Jul 26;382(3):779–789. doi: 10.1016/j.jmb.2008.07.054

Table 2.

Primer pairings used for amplification of gene segments

Primer pairings	Products	Targets
FR1F- H1R	FR1-CDR1-FR2^a	CDR1

H2F1- H2R1	FR2^a-CDR2-FR3^a	VH1-*^b (except VH1-24^c, VH1-F)
H2R2		VH6-1
H2R5		VH7-*
H2F2- H2R3		VH2-*
H2F3- H2R1		VH1-24, VH1-F
H2R2		VH3-*
H2R3		VH4-*
H2R4		VH5-*

H3F1- H3R	FR3^a-CDR3-FR4	VH3-9, VH3-20, VH3-43
H3F2		VH1-45, VH2-, VH5-, VH7-81
H3F3		Other than above

FR3F- FR3R	FR3^a	FR3

^a

These products partially cover FRs.

^b

All members in the group of VH germlines

^c

Sub-group within the group of VH germlines.