Table 2.
Syncytia formation promoted by mutant Mo-MuLV envelope proteins
| Mutant envelope protein | Syncytia formationa |
|---|---|
| W606A | 124 ± 28 |
| W606G | 156 ± 45 |
| W606E | 90 ± 20 |
| W606S | 76 ± 14 |
| W606I | 104 ± 20 |
| W606F | 102 ± 28 |
| W606S (full-length cytoplasmic domain) | 8 ± 3 |
| F607A | 72 ± 33 |
| F607G | 104 ± 60 |
| F607V | <0.25 |
| F607W | 120 ± 28 |
| F607Y | 111 ± 32 |
| G616A | 61 ± 19 |
| G616V | 70 ± 5 |
| G616P | 121 ± 48 |
| P617A | 2 ± 1 |
| P617G | <0.25 |
| P617V | 12 ± 6 |
| Wild-type with full-length cytoplasmic domain | <0.25 |
| Wild-type with truncated cytoplasmic domain | 100 |
NIH 3T3 cells were transiently transfected with plasmids encoding the mutant envelope proteins lacking the last 16 cytoplasmic amino acids. The number of syncytia and the number of nuclei in syncytia were counted 36 h after transfection.
a
The numbers are normalized to the percentage of nuclei in syncytia formed as a result of expression of the wild-type envelope protein with a truncated cytoplasmic domain (8.3%).