Table 3.
Syncytia formation promoted by Mo-MuLV envelope proteins expressed in 293T cells
| Mutant envelope proteins | Syncytia formationa |
|---|---|
| P617A | 3 ± 1 |
| P617G | <0.25 |
| P617V | 6 ± 1 |
| Wild-type with full-length cytoplasmic domain | <0.25 |
| Wild-type with truncated cytoplasmic domain | 100a |
293T cells were transiently transfected with plasmids encoding the mutant envelope proteins lacking the last 16 cytoplasmic amino acids and cocultivated with NIH 3T3 cells. The number of syncytia and the number of nuclei in syncytia were counted 48 h after transfection.
a
The numbers are normalized to the percentage of nuclei in syncytia formed as a result of expression of the wild-type envelope protein with a truncated cytoplasmic domain (7%).