Table 4.
Cellular receptor binding by mutant Mo-MuLV envelope proteins
| Mutant envelope proteins | Interference |
|---|---|
| W606A | +++ |
| W606G | + |
| W606E | + |
| W606S | + |
| W606I | ++ |
| W606F | ++ |
| F607A | ++ |
| F607G | +++ |
| F607V | + |
| F607W | +++ |
| F607Y | +++ |
| G616A | +++ |
| G616V | +++ |
| G616P | ++ |
| P617A | +++ |
| P617G | +++ |
| P617V | +++ |
| NIH 3T3 cells not expressing envelope protein | − |
| Cells expressing wild-type envelope protein | +++ |
NIH 3T3 cells that were stably transfected with plasmids encoding the mutant envelope proteins were infected with virus produced by E86nlslacZ cells. Forty-eight hours after infection the cells were stained with X-gal, and the percentage of blue cells was calculated. The cells expressing the wild-type envelope protein were transduced at a level 13-fold lower than that of the NIH 3T3 cells.