Figure 1.

Sequence of RIP3. (A) The derived amino acid sequence of RIP3. The shaded areas contain a repetitive amino acid sequence that is often found in Dictyostelium ORFs and that is not thought to have an important function in the protein. RIP3 has a higher fraction of this sequence than other identified proteins. The domain that is homologous to the mammalian protein cloned, JC310, which was identified in a screen for mammalian genes that inhibit activated Ras function in yeast, is underlined. The two arrows mark the beginning of the ORFs of two different two-hybrid clones that were identified in our screen using activated mammalian Ha-Ras as bait. (B) Comparison of the amino acid homology between RIP3 and mammalian ORF JC310.