Figure 4.

Developmental phenotypes of wild-type and rip3 null cells. (A) The developmental phenotypes of wild-type KAx-3 cells. Mound formation is visible at 8 h. At 13 h, most of the aggregates have formed a tip, and at 16 h, the cells have formed a migrating slug or pseudoplasmodium. Cells have culminated by 24–26 h. (B) Developmental phenotype of rip3 null cells. At 8 h, the cells exhibit a small amount of rippling. At 24 h, very loose cellular associations are observed. Half or less of the cells are associated with the aggregates. Most cells show no sign of participation in aggregate formation.