Figure 10.
Influence of ANPK on the function of various AR mutants. (A) Structural features of AR mutants studied. (B) Effect of ANPK on AR mutants was examined in CV-1 cells by coexpressing rAR or the deletion mutants rAR▵40–147, rAR▵641–902, and rAR▵46–408/▵641–902 (0.5 μg of each pSG5 expression vector) in the presence of empty pFLAG-CMV2 expression vector (5 μg, open bars) or with pFLAG-ANPK(159–1191) (5 μg, solid bars) and 5 μg of pARE2-E1b-CAT reporter in the presence of 100 nM testosterone. Cells were transiently transfected using the calcium phosphate method. β-Gal expression plasmid, pCMVβ (2 μg/10-cm plate), was used to control for transfection efficiency. CAT activities are expressed relative to that of pSG5-rAR in the presence of testosterone (= 100), and the mean ± SE values of at least three independent experiments are given.