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. 1998 Sep;9(9):2527–2543. doi: 10.1091/mbc.9.9.2527

Figure 2.

Figure 2

Expression of ANPK. (A) Northern blot of RNA from rat tissues. Polyadenylated RNA samples (5 μg/lane) from testis (1), prostate (2), and brain (3). (B) Northern blot of poly(A)+ RNA (2 μg/lane) from various human tissues. Human multiple tissue Northern blot II (CLONTECH) contains RNA from spleen (1), thymus (2), prostate (3), testis (4), small intestine (5), colon (6), and peripheral blood leukocytes (7). Both blots were hybridized with 32P-labeled ANPK cDNA as described in MATERIALS AND METHODS. To check the integrity of the RNA samples, the rat blot was reprobed with rat γ-actin and the human blot with human β-actin cDNA, respectively. (C) Expression of ANPK protein in various cell lines. Fifteen micrograms of whole cell extracts from PC12 (1), N2A (2), LNCaP (3), CHO (4, 5), PC-3 (6), CV-1 (7), and COS-1 cells transfected with an ANPK expression vector (8), and mock-transfected COS-1 cells (9), or 2 μl of ANPK(1–1191) (10) and ANPK(159–1191) (11) produced by translation in vitro using reticulocyte lysate system were separated on 7.5% polyacrylamide gel under denaturing conditions and immunoblotted using rabbit antiserum against GST-ANPK(766–920).