Figure 8.

Colocalization of AR and ANPK in CV-1 cell nuclei. CV-1 cells seeded on glass cover slips on 10-cm plastic plates were cotransfected with 2.5 μg of FLAG-ANPK(2–1191) expression vector and 1 μg of rAR vector using DOTAP transfection reagent. Double immunofluorescence labeling was performed using anti-FLAG M2 monoclonal antibody and rabbit K183 antiserum for rAR as described in MATERIALS AND METHODS and analyzed by using a Bio-Rad MRC-1024 confocal laser scanning system connected to a Zeiss axiovert 100/135 microscope. Both ANPK (A, red) and AR (B, green) are present in nuclear dots, and the majority of the two proteins colocalize as revealed by the resulting yellow image following superimposition of A and B.