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. 2008 Sep 12;415(Pt 1):135–144. doi: 10.1042/BJ20080435

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© 2008 The Author(s) The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Non-Commercial Licence (http://creativecommons.org/licenses/by-nc/2.5/) which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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(A) EK activity was measured spectrophotometrically as a function of pH as described in the Experimental section. Results are means±S.D. of three measurements. (B, C) Determination of TbEK1 Michaelis–Menten constants for ethanolamine and ATP (insets are Lineweaver–Burk plots). (B) ATP concentration was held constant (5 mM), while ethanolamine concentration was varied. (C) Ethanolamine concentration was held constant (2 mM), while ATP concentration was varied.