Figure 6. Analysis of PtdCho formation by TbC/EK2.

(A) Reaction catalysed by TbC/EK2. Cho, choline. (B) The substrate ethanolamine (EtN) and the product PtdEtn of the reaction were separated by HPTLC as described in the Experimental section. Lanes 1–7, ninhydrin detection. Lane 1, ethanolamine standard; lane 2, PtdEtn standard; lane 3, standard reaction with TbC/EK2; lanes 4–6, no Mg²⁺, no ethanolamine or no ATP added to the reaction mixture respectively (negative controls). (C) The substrate choline (Cho) and the product PtdCho of the reaction were separated by HPTLC as described in the Experimental section. Lanes 1–6, autoradiography detection; lane 7, detection by iodine staining. Lane 1, [³H]choline standard; lane 2, standard reaction with TbC/EK2; lanes 3–6, no enzyme, no Mg²⁺, no [³H]-choline or no ATP added to the reaction mixture respectively (negative controls); lane 7, PtdCho standard visualized by iodine staining.