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. Author manuscript; available in PMC: 2008 Sep 24.
Published in final edited form as: J Biol Chem. 2003 Feb 17;278(18):16356–16364. doi: 10.1074/jbc.M301255200

Fig. 3. SDS Gel electrophoresis of fractions generated during the purification of LpcC.

Fig. 3

Lanes 1 and 2 contain 10 μg of protein from membranes and solubilized membranes, respectively, of BLR(DE3)/pLysS/pMKHN. Lane 3 contains 1.0 μg of LpcC after the nickel affinity column. Much of the overexpressed LpcC protein is not solubilized by Triton X-100, most likely because it is trapped in small inclusion bodies. However, about half of the mannosyl transferase activity present in whole membranes is recovered in the Triton X-100-solubilized fraction (see Table I).