Fig. 4. Sugar nucleotide specificity of purified LpcC.
Reactions were carried out under standard conditions at pH 7.5 using a 1 mM concentration of each of the sugar nucleotides, as indicated. Purified enzyme was used at 5 μg/ml. The incubation was carried out at 30 °C for 30 min. Under conditions where product evolution is linear with time (data not shown), the very slow reactions with UDP-glucose and UDP-galactose are not detectable.
