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. Author manuscript; available in PMC: 2008 Sep 24.
Published in final edited form as: J Biol Chem. 2004 Oct 15;279(53):55780–55791. doi: 10.1074/jbc.M406480200

Fig. 2. Detection of 1-phosphatase activity in membranes of wild type strains of H. pylori.

Fig. 2

Membranes from either E. coli wild type strain W3110 or H. pylori wild type strains (26695 and J99) were assayed for 1-phosphatase activity using assay conditions described under “Experimental Procedures.” The protein concentration was 1.0 mg/ml, and assays were carried out for both 30 min (A) and 3 h (B) at 30 °C. Each reaction contained 5 μM 4′-32P-lipid IVA substrate. Reaction products were separated by TLC and detected with PhosphorImager analysis. Removal of the 1-phosphate group was indicated by conversion of the 4′-32P-lipid IVA substrate to a faster migrating species (26).