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. Author manuscript; available in PMC: 2008 Sep 24.
Published in final edited form as: J Biol Chem. 2003 Feb 17;278(18):16365–16371. doi: 10.1074/jbc.M301256200

Fig. 7. Glycosylations of Kdo2-lipid IVA catalyzed by membranes of E. coli expressing lpsB behind the T7lac promoter.

Fig. 7

Reactions were carried out under standard conditions at pH 7.5 using a 1 mM concentration of each of the sugar nucleotides, as indicated. Enzyme was used at 0.02 mg/ml. The incubation was carried out at 30 °C for 60 min. A, membranes of BLR(DE3)/pLysS/pET28b; B, membranes of BLR(DE3)/pLysS/pMKRM.