Table I.
Sinorhizobium meliloti strains and plasmids used in this study
| Bacterial strains | Genotype | LPS properties | Source or reference |
|---|---|---|---|
| S. meliloti 1021 | Parental wild type | Full-length | Ref. 25 |
| S. meliloti 2011 | Parental wild type | Full-length | Ref. 26 |
| S. meliloti 6963 | lpsB::Tn5 | Truncated inner core | Ref. 8 |
| S. meliloti 6963-B | S. meliloti 6963 (pJBlpsSme) | Full-length | This work |
| S. meliloti 6963-C | S. meliloti 6963 (pPN120) | Truncated as in 6963 | This work |
| Plasmids | Relevant characteristics | ||
|
| |||
| pJB3Tc19 | Derivative of the minimal replicon of plasmid RK2, Apr, Tcr | Ref. 27 | |
| pAL100 | pACYC184-Gm-mob carrying in Dral a 5.5-kb SstI DNA fragment from S. meliloti 2011 harboring lpsB | Ref. 8 | |
| pJB1psSme | pJB3Tc19 carrying a 4.9-kb SstI-HindIII fragment with the region greA-lpsB-lpsE-lpsD from S. meliloti 2011 cloned into the polylinker of the vector between EcoRI-HindIII, Kmr | Ref. 7 | |
| pPN120 | pLAFR-1 with a 4.4 kb EcoRI fragment containing greA, lpcC, dctA, and the 53 region of dctB from R. leguminosarum bv. viciae, Tcr | Refs. 4 and 7 | |