Figure 5. Rates of MTSEA-Biotin modification ofα₁H101C.

Representative GABA (EC₁₅) and GABA + FLZM (EC₉₅) current responses from oocytes expressing α₁H101Cβ₂γ₂ receptors (A and B) and α₁H101Cβ₂ receptors (C) before and after 10 sec applications of MTSEA-Biotin (arrows). Decreases in FLZM potentiation of I-GABA (A) and increases in I-GABA (B and C) were plotted versus cumulative MTSEA-Biotin exposure. In A, data were normalized to FLZM potentiation measured before MTSEA-Biotin exposure (t=0 sec). In B and C, data were normalized to I-GABA measured before MTSEA-Biotin exposure. The data were fit with single exponential functions and the calculated second order rate constants (k₂) were determined as described in “Experimental Procedures”. Data points represent the mean +/− SEM from at least five independent experiments. k₂ values are summarized in Table 2.