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. 1983 Aug;47(2):367–369. doi: 10.1128/jvi.47.2.367-369.1983

Measurement of repair patch size by quantitation of nucleotides excised during DNA repair in vivo.

E H Radany, E C Friedberg
PMCID: PMC255269  PMID: 6352957

Abstract

Escherichia coli uvr- cells, prelabeled in their DNA, were infected with phage T4 denV+ or T4 denV- under conditions that preclude phage-mediated degradation of the bacterial chromosome. Measurement of the distribution of acid-soluble radioactivity between pyrimidine dimers and nondimer nucleotides in cell extracts yielded calculated estimates of the average size of excision repair tracts that are in good agreement with the size of repair patches determined by others using direct measurement of repair synthesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cooper P. Excision-repair in mutants of Escherichia coli deficient in DNA polymerase I and/or its associated 5' leads to 3' exonuclease. Mol Gen Genet. 1977 Jan 7;150(1):1–12. doi: 10.1007/BF02425319. [DOI] [PubMed] [Google Scholar]
  2. Demple B., Linn S. DNA N-glycosylases and UV repair. Nature. 1980 Sep 18;287(5779):203–208. doi: 10.1038/287203a0. [DOI] [PubMed] [Google Scholar]
  3. Gordon L. K., Haseltine W. A. Quantitation of cyclobutane pyrimidine dimer formation in double- and single-stranded DNA fragments of defined sequence. Radiat Res. 1982 Jan;89(1):99–112. [PubMed] [Google Scholar]
  4. Grafstrom R. H., Park L., Grossman L. Enzymatic repair of pyrimidine dimer-containing DNA. A 5' dimer DNA glycosylase: 3'-apyrimidinic endonuclease mechanism from Micrococcus luteus. J Biol Chem. 1982 Nov 25;257(22):13465–13474. [PubMed] [Google Scholar]
  5. Haseltine W. A., Gordon L. K., Lindan C. P., Grafstrom R. H., Shaper N. L., Grossman L. Cleavage of pyrimidine dimers in specific DNA sequences by a pyrimidine dimer DNA-glycosylase of M. luteus. Nature. 1980 Jun 26;285(5767):634–641. doi: 10.1038/285634a0. [DOI] [PubMed] [Google Scholar]
  6. Kuemmerle N., Ley R., Masker W. Analysis of resynthesis tracts in repaired Escherichia coli deoxyribonucleic acid. J Bacteriol. 1981 Aug;147(2):333–339. doi: 10.1128/jb.147.2.333-339.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kutter E., Beug A., Sluss R., Jensen L., Bradley D. The production of undegraded cytosine-containing DNA by bacteriophage T4 in the absence of dCTPase and endonucleases II and IV, and its effects on T4-directed protein synthesis. J Mol Biol. 1975 Dec 25;99(4):591–607. doi: 10.1016/s0022-2836(75)80174-4. [DOI] [PubMed] [Google Scholar]
  8. McMillan S., Edenberg H. J., Radany E. H., Friedberg R. C., Friedberg E. C. den V gene of bacteriophage T4 codes for both pyrimidine dimer-DNA glycosylase and apyrimidinic endonuclease activities. J Virol. 1981 Oct;40(1):211–223. doi: 10.1128/jvi.40.1.211-223.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Nakabeppu Y., Sekiguchi M. Physical association of pyrimidine dimer DNA glycosylase and apurinic/apyrimidinic DNA endonuclease essential for repair of ultraviolet-damaged DNA. Proc Natl Acad Sci U S A. 1981 May;78(5):2742–2746. doi: 10.1073/pnas.78.5.2742. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Nakabeppu Y., Yamashita K., Sekiguchi M. Purification and characterization of normal and mutant forms of T4 endonuclease V. J Biol Chem. 1982 Mar 10;257(5):2556–2562. [PubMed] [Google Scholar]
  11. Pawl G., Taylor R., Minton K., Friedberg E. C. Enzymes involved in thymine dimer excision in bacteriophage T4-infected Escherichia coli. J Mol Biol. 1976 Nov;108(1):99–109. doi: 10.1016/s0022-2836(76)80097-6. [DOI] [PubMed] [Google Scholar]
  12. Radany E. H., Friedberg E. C. A pyrimidine dimer-DNA glycosylase activity associated with the v gene product of bacterophage T4. Nature. 1980 Jul 10;286(5769):182–185. doi: 10.1038/286182a0. [DOI] [PubMed] [Google Scholar]
  13. Radany E. H., Friedberg E. C. Demonstration of pyrimidine dimer-DNA glycosylase activity in vivo: bacteriophage T4-infected Escherichia coli as a model system. J Virol. 1982 Jan;41(1):88–96. doi: 10.1128/jvi.41.1.88-96.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Seawell P. C., Smith C. A., Ganesan A. K. den V gene of bacteriophage T4 determines a DNA glycosylase specific for pyrimidine dimers in DNA. J Virol. 1980 Sep;35(3):790–796. doi: 10.1128/jvi.35.3.790-796.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Warner H. R., Christensen L. M., Persson M. L. Evidence that the UV endonuclease activity induced by bacteriophage T4 contains both pyrimidine dimer-DNA glycosylase and apyrimidinic/apurinic endonuclease activities in the enzyme molecule. J Virol. 1981 Oct;40(1):204–210. doi: 10.1128/jvi.40.1.204-210.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Warner H. R., Snustad P., Jorgensen S. E., Koerner J. F. Isolation of bacteriophage T4 mutants defective in the ability to degrade host deoxyribonucleic acid. J Virol. 1970 Jun;5(6):700–708. doi: 10.1128/jvi.5.6.700-708.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Yarosh D. B., Rosenstein B. S., Setlow R. B. Excision repair and patch size in UV-irradiated bacteriophage T4. J Virol. 1981 Nov;40(2):465–471. doi: 10.1128/jvi.40.2.465-471.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]

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