Figure 1.

TPA- and HGF/SF-induced reorganization of the actin cytoskeleton in wt MDCK cells. wt MDCK cells were incubated in DMEM containing 10% FCS for 24 h. After the incubation, the cells were stimulated with none (a and f), 100 nM TPA (b–e and g–j), or 20 ng/ml HGF/SF (k–r). The cells were fixed at 15 min (b and g), 1 h (c and h), 2 h (d and i), or 18 h (e and j) after TPA stimulation, and at 2 h (k and o), 4 h (l and p), 6 h (m and q), or 18 h (n and r) after HGF/SF stimulation, double stained with rhodamine-phalloidin (a–e and k–n) or the V115 anti-vinculin mAb (f–j and o–r), and analyzed by confocal microscopy. Confocal images are shown at the basal levels. The results shown are representative of three independent experiments. Bar, 10 μm. An arrowhead in panel i indicates the staining of vinculin at the newly formed focal adhesions. An arrow in panel j indicates the dot-like staining of vinculin that is located at the sites different from the focal adhesions.