Figure 3.

Inhibition of the TPA-induced disassembly of stress fibers and focal adhesions in sMDCK-RhoDA cells. sMDCK-RhoDA cells were incubated in DMEM containing 10% FCS for 24 h. After the incubation, the cells were stimulated with none (a and d) or 100 nM TPA (b, c, e, and f). The cells were fixed at 15 min (b and e) or 2 h (c and f) after TPA stimulation, double stained with rhodamine-phalloidin (a–c) or the V115 anti-vinculin mAb (d–f), and analyzed by confocal microscopy. Confocal images are shown at the basal levels. The results shown are representative of three independent experiments. Bar, 10 μm. Arrowheads in panels b and c indicate the reduced peripheral bundles.