Figure 6.

Inhibition by Rab GDI of the TPA-induced reassembly of stress fibers and focal adhesions. wt MDCK cells were incubated in DMEM containing 10% FCS for 24 h. After the incubation, the cells were microinjected with 5 mg/ml His6-Rab GDI plus 5 mg/ml rat IgG. At 30 min after the microinjection, the cells were stimulated with none (a and e) or 100 nM TPA (b–d and f–h). At 15 min (b and f) or 2 h (c, d, g, and h) after TPA stimulation, the cells were fixed, stained with rhodamine-phalloidin (a–c) or the V115 anti-vinculin mAb (d), and analyzed by confocal microscopy. The microinjected cells are shown by the staining of microinjected rat IgG (e–h). Confocal images are shown at the basal levels. The results shown are representative of three independent experiments. Bars, 10 μm.