Figure 9.

Inhibition of the TPA-induced reassembly of stress fibers and focal adhesions in sMDCK-Rab5DN cells. sMDCK-Rab5DN-10 (a–f), -Rab11DA-3 (g–l), or -Rab11DN-5 (m–r) cells were incubated in DMEM containing 10% FCS for 24 h. The cells were stimulated with none (a, d, g, j, m, and p) or 100 nM TPA (b, c, e, f, h, i, k, l, n, o, q, and r). At 15 min (b, e, h, k, n, and q) or 2 h (c, f, i, l, o, and r) after TPA stimulation, the cells were fixed, double stained with rhodamine-phalloidin (a–c, g–i, and m–o) or the V115 anti-vinculin mAb (d–f, j–l, and p–r), and analyzed by confocal microscopy. Confocal images are shown at the basal levels. The results shown are representative of three independent experiments. Bar, 10 μm.