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. 1999 Sep;10(9):2891–2904. doi: 10.1091/mbc.10.9.2891

Figure 2.

Figure 2

Immunogold localization of αβ-Ii complexes after 2 h of 19°C incubation and upon shifting to 37°C. Ultrathin cryosections of Laz cells were immunogold labeled with an anti-αβ-DR rabbit serum (PAG-10) and a rabbit Ab directed against the C-terminal domain of the Ii chain (ICC5) (PAG-15). (A) Laz cells incubated at 19°C for 2h. Intense labeling is observed for the Ii chain (PAG 15) and MHC class II (PAG 10) in the Golgi apparatus. (B and C) Cells incubated at 19°C for 2 h and then shifted to 37°C for 45 min with Ii chain (PAG 15) and MHC class II (PAG 10) are visualized in electron-lucent vesicles and tubules apposed to the Golgi apparatus (B) as well as in endosomes (C). Bar, 100 nm.