Figure 4.

Early endosome inactivation has no effect on ligand internalization but reduces their degradation. A6B9 cells were processed for early endosome inactivation. Control cells were submitted to the same treatment as cross-linked cells but were incubated with non–HRP-coupled Tf before testing FcγRIIb2-mediated ligand internalization and degradation. (A) ¹²⁵I-2.4G2 kinetics of internalization in both control (black circles) and cross-linked cells (black squares); (b) Degradation of ¹²⁵I-DNP-BSA-anti-DNP ICs determined by measuring the percentage of 20% TCA-soluble radioactivity. Each result shows the average obtained with four independent experiments.