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. 1999 Sep;10(9):2891–2904. doi: 10.1091/mbc.10.9.2891

Figure 8.

Figure 8

Figure 8

Immunogold localization of Ii chain in cross-linked cells. Laz cells were incubated at 19°C for 2 h in the presence of Tf-HRP and processed for early endosomes inactivation as before. The cells were then chased at 37°C for 45 min to induce accumulation of MHC class II in compartments en route to the endocytic pathway. Ultrathin cryosections were immunogold labeled with the anti-Ii chain Ab ICC5 (PAG15) and with the anti-γ-adaptin mAb (PAG10; upper panel) or with the anti-46-kDa M6PR Ab (PAG10; lower panel). Upper panel, note that the Ii-positive vesicles (PAG 15) are distinct from the γ-adaptin (PAG 10)–labeled vesicles. Lower panel, the vesicles and tubulovesicular structures (inset) labeled with the anti-Ii antibody are not labeled with the anti-46-kDa M6PR antibody. Bar, 200 nm.